Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8)

Product Name

Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8)

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SKU/Catalog Number

M02571

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8) (Catalog# M02571). Tested in IHC, FACS, WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02571)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM14A8

Isotype

IgG1 Kappa

Immunogen

A partial length recombinant MBD3 protein (amino acids 1-290)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02571 is reactive to Mbd3 in Human

Applications

M02571 is guaranteed for IHC, WB, FACS Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.168kDa

Background of Mbd3

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml
IHC: 5 µg/ml, FACS analysis: 0.5 µg/10^6 cells

Validation Images & Assay Conditions

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Immunohistochemical analysis of MBD3 in Squamous cell carcinoma of Lungs using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.

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Immunohistochemical analysis of MBD3 in Squamous cell carcinoma of Lungs using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.

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Immunohistochemical analysis of MBD3 in human Colon tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.

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Immunohistochemical analysis of MBD3 in human Spleen tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.

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Immunohistochemical analysis of MBD3 in human Testis tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.

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Immunohistochemical analysis of MBD3 in human Kidney tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.

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Immunohistochemical analysis of MBD3 in human Testis tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.

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Immunohistochemical analysis of MBD3 in human breast tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.

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Western blot analysis of MBD3 (and MBD2). Anti-MBD3 (and MBD2) antibody (Clone: ABM14A8) was used at 2 μg/ml on A431, K562, A375 and HEK293 lysates.

Gene/Protein Information For Mbd3 (Source: Uniprot.org, NCBI)

Alternative Names

MBD3; methyl-CpG binding domain protein 3; methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 Mbd3|AI181826, AU019209|methyl-CpG binding domain protein 3|methyl-CpG-binding domain protein 3|methyl-CpG-binding protein MBD3

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In this infographic, you will see the following information for Mbd3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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